Kolawole, OM, Seriki, AA, Irekeola, AA et al. (2 more authors) (2017) Dengue virus and malaria concurrent infection among febrile subjects within Ilorin metropolis, Nigeria. Journal of Medical Virology, 89 (8). pp. 1347-1353. ISSN 0146-6615
Abstract
Dengue is a mosquito-borne disease of public health importance. It is caused by four serotypes of Dengue virus (i.e, DENV-1, -2, -3, and -4). As a result of practices that are conducive for mosquito breading, its vector is widespread in Nigeria and this could result to possible DENV outbreaks in Nigeria and beyond. This study aimed to assess the recency of DENV infection as well as occurrence of DENV and Malaria co-infections within Ilorin, Nigeria. Blood samples were obtained from 176 febrile subjects and analyzed using Enzyme Linked Immunosorbent Assay (ELISA) for the presence of DENV antibodies. Malaria infection was detected using a rapid diagnostic test kit for malaria parasites. Malaria and DENV (IgM positive) co-infected samples were further subjected to RT-qPCR analysis. A seroprevalence of 46.0% was recorded for anti-DENV IgM antibodies and 2.84% for concurrent Dengue and malaria infections. Out of 95 IgM negative samples, 48 were found to be positive for DENV IgG antibodies. Eleven (6.25%) samples were confirmed DENV positive following RT-qPCR. The CT values of the amplicons were between 19.0 and 20.0. DENV serotype 2 dominated the study, while serotype 3 and 4 were equally distributed. Based on the high seroprevalence of DENV obtained in this study, there is a high possibility of experiencing Dengue virus outbreak in Ilorin, Nigeria, not neglecting the fast geographical spread of the vector. Therefore, surveillance and intensive vector control program should be instituted.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Wiley Periodicals, Inc. This is the peer reviewed version of the following article: Kolawole OM, Seriki AA, Irekeola AA, Bello KE, Adeyemi OO. Dengue virus and malaria concurrent infection among febrile subjects within Ilorin metropolis, Nigeria. J Med Virol. 2017; 89: 1347–1353, which has been published in final form at https://doi.org/10.1002/jmv.24788. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Jun 2017 09:03 |
Last Modified: | 15 Feb 2018 01:38 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/jmv.24788 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:117352 |