Evans, R.J. orcid.org/0000-0003-0678-6510, Voelz, K., Johnston, S.A. orcid.org/0000-0002-3429-9536 et al. (1 more author) (2017) Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages. In: Botelho, R., (ed.) Phagocytosis and Phagosomes. Methods in Molecular Biology (1519). Springer New York , pp. 349-357. ISBN 978-1-4939-6579-3
Abstract
Flow cytometry is a powerful analytical technique, which is increasingly being used to study the interaction between host cells and intracellular pathogens. Flow cytometry is capable of measuring a greater number of infected cells within a sample compared to alternative techniques such as fluorescence microscopy. This means that robust quantification of rare events during infection is possible. Our lab and others have developed flow cytometry methods to study interactions between host cells and intracellular pathogens, such as Cryptococcus neoformans, to quantify phagocytosis, intracellular replication, and non-lytic expulsion or "vomocytosis" from the phagosome. Herein we describe these methods and how they can be applied to the study of C. neoformans as well as other similar intracellular pathogens.
Metadata
Item Type: | Book Section |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © 2017 Springer. This is an author produced version of a book chapter subsequently published in Phagocytosis and Phagosomes. Uploaded in accordance with the publisher's self-archiving policy. The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-6581-6_24. |
Keywords: | Cryptococcus neoformans; Cryptococcus gattii; Flow cytometry; Macrophage; Infection; Mycology |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease The University of Sheffield > Sheffield Teaching Hospitals |
Funding Information: | Funder Grant number MEDICAL RESEARCH COUNCIL C0494 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 15 Jun 2017 13:39 |
Last Modified: | 05 Nov 2017 21:18 |
Published Version: | https://doi.org/10.1007/978-1-4939-6581-6_24 |
Status: | Published |
Publisher: | Springer New York |
Series Name: | Methods in Molecular Biology |
Refereed: | Yes |
Identification Number: | 10.1007/978-1-4939-6581-6_24 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:117211 |