Wilton, D.J., Ghosh, M., Chary, K.V.A. et al. (2 more authors) (2008) Structural change in a B-DNA helix with hydrostatic pressure. Nucleic Acids Research, 36 (12). pp. 4032-4037. ISSN 0305-1048
Abstract
Study of the effects of pressure on macromolecular structure improves our understanding of the forces governing structure, provides details on the relevance of cavities and packing in structure, increases our understanding of hydration and provides a basis to understand the biology of high-pressure organisms. A study of DNA, in particular, helps us to understand how pressure can affect gene activity. Here we present the first high-resolution experimental study of B-DNA structure at high pressure, using NMR data acquired at pressures up to 200 MPa (2 kbar). The structure of DNA compresses very little, but is distorted so as to widen the minor groove, and to compress hydrogen bonds, with AT pairs compressing more than GC pairs. The minor groove changes are suggested to lead to a compression of the hydration water in the minor groove.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2008 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | EGG-WHITE LYSOZYME; NUCLEIC-ACIDS; CRYSTAL-STRUCTURE; CHEMICAL-SHIFTS; CONFORMATIONAL-CHANGES; VOLUMETRIC PROPERTIES; ESCHERICHIA-COLI; PROTEIN-G; CRYSTALLOGRAPHY; HYDRATION |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 06 Jun 2017 09:44 |
Last Modified: | 06 Jun 2017 09:44 |
Published Version: | http://doi.org/10.1093/nar/gkn350 |
Status: | Published |
Publisher: | Oxford University Press |
Refereed: | Yes |
Identification Number: | 10.1093/nar/gkn350 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:117205 |