Rossman, Maxim, Greive, Sandra Joy Muriatu orcid.org/0000-0001-6067-2632, Moschetti, Tommasso et al. (2 more authors) (2017) Development of a multipurpose scaffold for the display of peptide loops. Protein Engineering, Design and Selection. pp. 419-430. ISSN 1741-0134
Abstract
Protein–protein interactions (PPIs) determine a wide range of biological processes and analysis of these dynamic networks is increasingly becoming a mandatory tool for studying protein function. Using the globular ATPase domain of recombinase RadA as a scaffold, we have developed a peptide display system (RAD display), which allows for the presentation of target peptides, protein domains or full-length proteins and their rapid recombinant production in bacteria. The design of the RAD display system includes differently tagged versions of the scaffold, which allows for flexibility in the protein purification method, and chemical coupling for small molecule labeling or surface immobilization. When combined with the significant thermal stability of the RadA protein, these features create a versatile multipurpose scaffold system. Using various orthogonal biophysical techniques, we show that peptides displayed on the scaffold bind to their natural targets in a fashion similar to linear parent peptides. We use the examples of CK2β/CK2α kinase and TPX2/Aurora A kinase protein complexes to demonstrate that the peptide displayed by the RAD scaffold can be used in PPI studies with the same binding efficacy but at lower costs compared with their linear synthetic counterparts.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author 2017. Published by Oxford University Press. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 30 May 2017 15:45 |
Last Modified: | 21 Jan 2025 17:26 |
Published Version: | https://doi.org/10.1093/protein/gzx017 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1093/protein/gzx017 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:117116 |