Pensabene, V orcid.org/0000-0002-3352-8202, Crowder, SW, Balikov, DA et al. (2 more authors) (2016) Optimization of electrospun fibrous membranes for in vitro modeling of blood-brain barrier. In: Proceedings of the IEEE 38th Annual International Conference of the Engineering in Medicine and Biology Society (EMBC 2016). EMBC 2016, 16-20 Aug 2016, Orlando, Florida, USA. IEEE , pp. 125-128. ISBN 9781457702204
Abstract
The blood-brain barrier (BBB) plays a critical role in brain homeostasis at the cellular and global level. Mimicking the selective permeability and transport properties of the BBB to specific molecules and cells remains a significant challenge towards the development of a physiologically relevant in vitro BBB model. In this study, we developed electrospun poly (ϵ-caprolactone) (PCL) and polyethylene glycol (PEG) copolymer membranes that supported different cellular components of the neurovascular unit including human-derived endothelial cells, pericytes and astrocytes. Comparative analyses of thickness, morphology, biocompatibility and permeability of membranes were also conducted. We found that collagen coated 4%PEG-96%PCL membranes supported the growth of a confluent and tight endothelium confirmed by transendothelial electrical resistance measurements (TEER). Based on fabrication process and reported results, we finally discuss the adoption of these electrospun fiber membranes for in vitro and on-a-chip human BBB models.
Metadata
Item Type: | Proceedings Paper |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2016, IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. |
Keywords: | Permeability, Extraterrestrial measurements, Biomembranes, Polymers, Morphology, In vitro, Biomedical engineering |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 23 May 2017 15:22 |
Last Modified: | 23 Jan 2018 03:04 |
Status: | Published |
Publisher: | IEEE |
Identification Number: | 10.1109/EMBC.2016.7590656 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:116751 |