Zhihua, L, Xuetao, H, Jiyong, S et al. (6 more authors) (2016) Bacteria counting method based on polyaniline/bacteria thin film. Biosensors and Bioelectronics, 81. pp. 75-79. ISSN 0956-5663
Abstract
A simple and rapid bacteria counting method based on polyaniline (PANI)/bacteria thin film was proposed. Since the negative effects of immobilized bacteria on the deposition of PANI on glass carbon electrode (GCE), PANI/bacteria thin films containing decreased amount of PANI would be obtained when increasing the bacteria concentration. The prepared PANI/bacteria film was characterized with cyclic voltammetry (CV) technique to provide quantitative index for the determination of the bacteria count, and electrochemical impedance spectroscopy (EIS) was also performed to further investigate the difference in the PANI/bacteria films. Good linear relationship of the peak currents of the CVs and the log total count of bacteria (Bacillus subtilis) could be established using the equation Y=−30.413X+272.560 (R²=0.982) over the range of 5.3×10 ⁴ to 5.3×10 ⁸ CFU mL−1, which also showed acceptable stability, reproducibility and switchable ability. The proposed method was feasible for simple and rapid counting of bacteria.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Polyaniline; Bacteria counting; Thin film |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 01 Jun 2017 15:46 |
Last Modified: | 01 Jun 2017 15:46 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.bios.2016.02.022 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:116710 |