Tozer, G.M., Prise, V.E. and Cunningham, V.J. (2016) Quantitative estimation of tissue blood flow rate. Methods in Molecular Biology, 1430. pp. 265-281. ISSN 1064-3745
Abstract
The rate of blood flow through a tissue (F) is a critical parameter for assessing the functional efficiency of a blood vessel network following angiogenesis. This chapter aims to provide the principles behind the estimation of F, how F relates to other commonly used measures of tissue perfusion, and a practical approach for estimating F in laboratory animals, using small readily diffusible and metabolically inert radio-tracers. The methods described require relatively nonspecialized equipment. However, the analytical descriptions apply equally to complementary techniques involving more sophisticated noninvasive imaging. Two techniques are described for the quantitative estimation of F based on measuring the rate of tissue uptake following intravenous administration of radioactive iodo-antipyrine (or other suitable tracer). The Tissue Equilibration Technique is the classical approach and the Indicator Fractionation Technique, which is simpler to perform, is a practical alternative in many cases. The experimental procedures and analytical methods for both techniques are given, as well as guidelines for choosing the most appropriate method.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Springer Science+Business Media. This is an author produced version of a paper subsequently published in Methods in Molecular Biology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Backflux; Blood flow rate; Cannulation; Distribution volume; Indicator fractionation; Iodo-antipyrine; Partition coefficient; Radiotracer; Tissue equilibration |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 18 May 2017 15:11 |
Last Modified: | 03 Nov 2017 02:20 |
Published Version: | https://doi.org/10.1007/978-1-4939-3628-1_18 |
Status: | Published |
Publisher: | Humana Press |
Refereed: | Yes |
Identification Number: | 10.1007/978-1-4939-3628-1_18 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:116621 |