Ramu, V., Aute, S., Taye, N. et al. (7 more authors) (2017) Photo-Induced Cytotoxicity and Anti-Metastatic Activity of Ruthenium(II)-Polypyridyl Complexes Functionalized with Tyrosine or Tryptophan. Dalton Transactions, 46. pp. 6634-6644. ISSN 1477-9226
Abstract
The synergistic effect of oxygen, light, and photosensitizer (PS) has found applications in medicine for the treatment of cancer through photodynamic therapy (PDT). Induction of apoptosis to cancerous cells will prevent tumor metastasis that spreads cancer cells to the neighboring organs/tissues. Herein, we report the two apoptotic Ru(II)–polypyridyl complexes that are functionalized with pendant amino acid moieties tyrosine (1) and tryptophan (2), respectively. These two water soluble complexes were found to interact strongly (K1a = (1.18 ± 0.28) × 105 M−1 and K2a = (1.57 ± 0.77) × 105 M−1) with CT-DNA. Isothermal titration calorimetry (ITC) studies revealed that these complexes bind to CT-DNA through an entropically driven process. Both the complexes showed photo-induced cytotoxicity and exhibit apoptotic activity under photo-irradiation conditions. The comet assay indicated that these complexes can damage cellular DNA, which is attributed to the significant build-up of 1O2 level even on irradiation with low intensity light (10 J cm−2, λRange 450–480 nm). This photoinduced DNA damage and apoptosis in A549 cells was induced by reactive oxygen species (ROS) and occurred through up-regulation of apoptotic marker caspase-3. Control experiments under dark conditions revealed an insignificant cytotoxicity towards these cells for two photosensitive molecules.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Royal Society of Chemistry. This is an author produced version of a paper subsequently published in Dalton Transactions. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Funding Information: | Funder Grant number BRITISH COUNCIL (THE) SA07-0113 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 21 Apr 2017 11:23 |
Last Modified: | 19 Apr 2018 00:38 |
Published Version: | https://doi.org/10.1039/C7DT00670E |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Refereed: | Yes |
Identification Number: | 10.1039/C7DT00670E |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:115262 |