Beattie, Thomas R., Kapadia, Nitin, Nicolas, Emilien et al. (4 more authors) (2017) Frequent exchange of the DNA polymerase during bacterial chromosome replication. eLife. e21763. ISSN 2050-084X
Abstract
The replisome is a multiprotein machine that carries out DNA replication. In Escherichia coli, a single pair of replisomes is responsible for duplicating the entire 4.6 Mbp circular chromosome. In vitro studies of reconstituted E. coli replisomes have attributed this remarkable processivity to the high stability of the replisome once assembled on DNA. By examining replisomes in live E. coli with fluorescence microscopy, we found that the Pol III* subassembly frequently disengages from the replisome during DNA synthesis and exchanges with free copies from solution. In contrast, the DnaB helicase associates stably with the replication fork, providing the molecular basis for how the E. coli replisome can maintain high processivity and yet possess the flexibility to bypass obstructions in template DNA. Our data challenges the widely-accepted semidiscontinuous model of chromosomal replication, instead supporting a fully discontinuous mechanism in which synthesis of both leading and lagging strands is frequently interrupted.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017, Beattie et al. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Physics (York) |
Depositing User: | Pure (York) |
Date Deposited: | 11 Apr 2017 14:20 |
Last Modified: | 16 Oct 2024 13:40 |
Published Version: | https://doi.org/10.7554/eLife.21763 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.7554/eLife.21763 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:114957 |
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Filename: elife_21763_v2.pdf
Description: Frequent exchange of the DNA polymerase during bacterial chromosome replication