Qin, X, Engwer, C, Desai, S et al. (2 more authors) (2017) An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules. Colloids and Surfaces B: Biointerfaces, 149. pp. 358-368. ISSN 0927-7765
Abstract
We examined the interaction between chitosan-based nanocapsules (NC), with average hydrodynamic diameter ∼114–155 nm, polydispersity ∼0.127, and ζ-potential ∼+50 mV, and an E. coli bacterial quorum sensing reporter strain. Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) allowed full characterization and assessment of the absolute concentration of NC per unit volume in suspension. By centrifugation, DLS, and NTA, we determined experimentally a “stoichiometric” ratio of ∼80 NC/bacterium. By SEM it was possible to image the aggregation between NC and bacteria. Moreover, we developed a custom in silico platform to simulate the behavior of particles with diameters of 150 nm and ζ-potential of +50 mV on the bacterial surface. We computed the detailed force interactions between NC-NC and NC-bacteria and found that a maximum number of 145 particles might interact at the bacterial surface. Additionally, we found that the “stoichiometric” ratio of NC and bacteria has a strong influence on the bacterial behavior and influences the quorum sensing response, particularly due to the aggregation driven by NC.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Elsevier B.V. This is an author produced version of a paper published in Colloids and Surfaces B: Biointerfaces. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Nanocapsules; Chitosan; Quorum sensing; Bacteria; Interactions |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 11 Apr 2017 09:37 |
Last Modified: | 17 Oct 2017 23:33 |
Published Version: | https://doi.org/10.1016/j.colsurfb.2016.10.031 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.colsurfb.2016.10.031 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:114881 |