Talbot, J., Brion, R., Lamora, A. et al. (4 more authors) (2018) Connexin43 intercellular communication drives the early differentiation of human bone marrow stromal cells into osteoblasts. Journal of Cellular Physiology, 233 (2). pp. 946-957. ISSN 0021-9541
Abstract
Although it has been demonstrated that human bone marrow stromal cells (hBMSCs) express the ubiquitous connexin43 (Cx43) and form functional gap junctions, their role in the early differentiation of hBMSCs into osteoblasts remains poorly documented. Using in vitro assays, we show that Cx43 expression and gap junctional intercellular communication (GJIC) are increased during the differentiation of hBMSCs into osteoblasts, both at the protein and mRNA levels. Two independent procedures to reduce GJIC, a pharmacological approach with GJIC inhibitors (18α-glycyrrhetinic acid and Gap27 peptide) and a molecular approach using small interfering RNA against Cx43, demonstrated that the presence of Cx43 and functional junctional channels are essential to the ability of hBMSCs to differentiate into osteoblasts in vitro. In addition, a reduced GJIC decreases the expression of Runx2, the major transcription factor implicated in the control of osteoblast commitment and early differentiation of hBMSCs into osteoblasts, suggesting that GJIC mediated by Cx43 is implicated in this process. Together our results demonstrate that GJIC mediated by the Cx43 channels plays a central role throughout the differentiation of hBMSC into osteoblasts, from the early stages to the process of mineralization. This article is protected by copyright. All rights reserved
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 John Wiley & Sons, Inc. This is an author produced version of a paper subsequently published in Journal of Cellular Physiology. Uploaded in accordance with the publisher's self-archiving policy |
Keywords: | cell differentiation; connexin43; gap junction; human bone marrow stromal cells; intercellular communication; osteoblast |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) |
Funding Information: | Funder Grant number ECONOMIC & SOCIAL RESEARCH COUNCIL ES/R000247/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 06 Apr 2017 12:55 |
Last Modified: | 17 Jul 2023 15:34 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/jcp.25938 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:114525 |