van der Putten, MA, MacKenzie, LE orcid.org/0000-0002-8151-0525, Davies, AL et al. (4 more authors) (2017) A multispectral microscope for in vivo oximetry of rat dorsal spinal cord vasculature. Physiological Measurement, 38 (2). pp. 205-218. ISSN 0967-3334
Abstract
Quantification of blood oxygen saturation (SO2) in vivo is essential for understanding the pathogenesis of diseases in which hypoxia is thought to play a role, including inflammatory disorders such as multiple sclerosis (MS) and rheumatoid arthritis (RA). We describe a low-cost multispectral microscope and oximetry technique for calibration-free absolute oximetry of surgically exposed blood vessels in vivo. We imaged the vasculature of the dorsal spinal cord in healthy rats, and varied inspired oxygen (FiO2) in order to evaluate the sensitivity of the imaging system to changes in SO2. The venous SO2 was calculated as 67.8 ± 10.4% (average ± standard deviation), increasing to 83.1 ± 11.6% under hyperoxic conditions (100% FiO2) and returning to 67.4 ± 10.9% for a second normoxic period; the venous SO2 was 50.9 ± 15.5% and 29.2 ± 24.6% during subsequent hypoxic states (18% and 15% FiO2 respectively). We discuss the design and performance of our multispectral imaging system, and the future scope for extending this oximetry technique to quantification of hypoxia in inflamed tissue.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Institute of Physics and Engineering in Medicine. This is an author produced version of a paper published in Physiological Measurement. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | multispectral imaging; oximetry; spinal cord vasculature |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 30 Mar 2017 11:22 |
Last Modified: | 19 Jan 2018 01:38 |
Status: | Published |
Publisher: | IOP Publishing |
Identification Number: | 10.1088/1361-6579/aa5527 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:114252 |