Brooks, A.C. and Hwang, L.C. orcid.org/0000-0001-9533-1184 (2017) Reconstitutions of plasmid partition systems and their mechanisms. Plasmid, 91. ISSN 0147-619X
Abstract
Bacterial plasmid and chromosome segregation systems ensure that genetic material is efficiently transmitted to progeny cells. Cell-based studies have shed light on the dynamic nature and the molecular basis of plasmid partition systems. In vitro reconstitutions, on the other hand, have proved to be an invaluable tool for studying the minimal components required to elucidate the mechanism of DNA segregation. This allows us to gain insight into the biological and biophysical processes that enable bacterial cells to move and position DNA. Here, we review the reconstitutions of plasmid partition systems in cell-free reactions, and discuss recent work that has begun to challenge long standing models of DNA segregation in bacteria.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Elsevier. This is an author produced version of a paper subsequently published in Plasmid. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/) |
Keywords: | In vitro reconstitution; Plasmid partition; DNA segregation; Cell-free reaction; ParA ATPase; Diffusion ratchet |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Funding Information: | Funder Grant number ROYAL SOCIETY RG150776 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 27 Mar 2017 10:41 |
Last Modified: | 18 Mar 2018 01:39 |
Published Version: | https://doi.org/10.1016/j.plasmid.2017.03.004 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.plasmid.2017.03.004 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:114086 |
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