Twarock, Reidun orcid.org/0000-0002-1824-2003, Dykeman, Eric Charles, Shakeel, Shabih et al. (5 more authors) (2017) Genomic RNA folding mediates assembly of human parechovirus. Nature Communications. 5. ISSN 2041-1723
Abstract
Assembly of the major viral pathogens of the Picornaviridae family is poorly understood. Human parechovirus 1 is an example of such viruses that contains 60 short regions of ordered RNA density making identical contacts with the protein shell. We show here via a combination of RNA-based systematic evolution of ligands by exponential enrichment, bioinformatics analysis and reverse genetics that these RNA segments are bound to the coat proteins in a sequence-specific manner. Disruption of either the RNA coat protein recognition motif or its contact amino acid residues is deleterious for viral assembly. The data are consistent with RNA packaging signals playing essential roles in virion assembly. Their binding sites on the coat proteins are evolutionarily conserved across the Parechovirus genus, suggesting that they represent potential broad-spectrum anti-viral targets.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017, The Authors. |
Keywords: | Amino Acid Motifs,Base Pairing,Binding Sites,Capsid Proteins/genetics,Computational Biology,Conserved Sequence,Gene Expression,Genome, Viral,Humans,Models, Molecular,Parechovirus/genetics,Protein Binding,RNA Folding,RNA, Viral/genetics,Reverse Genetics,SELEX Aptamer Technique,Virion/genetics,Virus Assembly |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Mathematics (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Funding Information: | Funder Grant number THE ROYAL SOCIETY UNSPECIFIED EPSRC EP/K028286/1 |
Depositing User: | Pure (York) |
Date Deposited: | 06 Mar 2017 12:40 |
Last Modified: | 08 Feb 2025 00:22 |
Published Version: | https://doi.org/10.1038/s41467-016-0011-z |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1038/s41467-016-0011-z |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:113228 |
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Description: Genomic RNA folding mediates assembly of human parechovirus
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