Noy, A, Maxwell, A and Harris, SA (2017) Interference between Triplex and Protein Binding to Distal Sites on Supercoiled DNA. Biophysical Journal, 112 (3). pp. 523-531. ISSN 0006-3495
Abstract
We have explored the interdependence of the binding of a DNA triplex and a repressor protein to distal recognition sites on supercoiled DNA minicircles using MD simulations. We observe that the interaction between the two ligands through their influence on their DNA template is determined by a subtle interplay of DNA mechanics and electrostatics, that the changes in flexibility induced by ligand binding play an important role and that supercoiling can instigate additional ligand-DNA contacts that would not be possible in simple linear DNA sequences.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © 2017, The Author(s). This article is published under a Creative Commons Attribution 4.0 International (CC BY 4.0). |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Theoretical Physics (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 03 Mar 2017 11:45 |
Last Modified: | 05 Oct 2017 16:35 |
Published Version: | https://doi.org/10.1016/j.bpj.2016.12.034 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.bpj.2016.12.034 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:113136 |