Matthews, S., Tee, K.L., Rattray, N.J. et al. (5 more authors) (2017) Production of alkenes and novel secondary products by P450 OleT JE using novel H2O2-generating fusion protein systems. FEBS Letters, 591 (5). pp. 737-750. ISSN 0014-5793
Abstract
Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2O2) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase (AldO) is linked to OleTJE. AldO oxidizes polyols (including glycerol), generating H2O2 as a co-product and facilitating its use for efficient OleTJE-dependent fatty acid decarboxylation. AldO activity is regulatable by polyol substrate titration, enabling control over H2O2 supply to minimise oxidative inactivation of OleTJE and prolong activity for increased alkene production. We also use these fusion systems to generate novel products from secondary turnover of 2-OH and 3-OH myristic acid primary products, expanding the catalytic repertoire of OleTJE.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Wiley. This is an author produced version of a paper subsequently published in FEBS Letters. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | OleT JE; CYP152L1; cytochrome P450; alkene production; hydrogen peroxide; fusion enzymes OleT JE peroxygenase P450; alkene production; fusion enzymes |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 15 Feb 2017 16:34 |
Last Modified: | 30 Jun 2023 14:07 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/1873-3468.12581 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:112349 |