Fernandes, J., Gentile, P., Crawford, A. et al. (3 more authors) (2017) Substituted Borosilicate Glasses with Improved Osteogenic Capacity for Bone Tissue Engineering. Tissue Engineering - Part A, 23 (23-24). pp. 1331-1342. ISSN 1937-3341
Abstract
Borosilicate bioactive glasses (BBGs) have shown capacity to improve the new bone formation when compared to silicate bioactive glasses. Herein, we assessed the capacity of BBGs to induce the osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs), as a function of their substituted divalent cations (Mg2+, Ca2+, Sr2+). To this purpose, we synthesized BBG particles by melt quench. The cell viability, proliferation and morphology, (i.e. PrestoBlue®, PicroGreen®, and DAPI and Phalloidin stainings, respectively) as well as protein expression (of ALP, osteopontin and osteocalcin) of BM-MSCs in contact with the BBGs were evaluated for 21 days. We observed an enhanced expression of bone-specific proteins (ALP, OP and OC) and high mineralization of BM-MSCs under BBG-Mg and BBG–Sr conditioned osteogenic media for concentrations of 20 and 50 mg/ml with low cytotoxic effects. Moreover, BBG-Sr at a concentration of 50 mg/ml was able to increase the mineralization and expression of bone-specific proteins even under basal media conditions. These results indicated that the proposed BBGs improved the osteogenic differentiation of BM-MSCs. Therefore, showing their potential as relevant biomaterials for bone tissue regeneration, not only by bonding to bone tissue, but also by stimulating new bone formation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017, Mary Ann Liebert, Inc. This is an author produced version of a paper subsequently published in Tissue Engineering Part A. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Borosilicate glasses; strontium; mineralization; osteogenic induction; BM-MSCs |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Clinical Dentistry (Sheffield) |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) EP/K029592/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) UNSPECIFIED ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL (EPSRC) UNSPECIFIED |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 14 Feb 2017 16:12 |
Last Modified: | 26 Jun 2018 10:50 |
Published Version: | https://doi.org/10.1089/ten.tea.2016.0386 |
Status: | Published |
Publisher: | Mary Ann Liebert |
Refereed: | Yes |
Identification Number: | 10.1089/ten.tea.2016.0386 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:112219 |