Duval, C., Ali, M., Chaudhry, W.W. et al. (3 more authors) (2016) Factor XIII A-Subunit V34L Variant Affects Thrombus Cross-Linking in a Murine Model of Thrombosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 36 (2). pp. 308-316. ISSN 1079-5642
Abstract
Objective-Factor XIII (FXIII) cross-links fibrin upon activation by thrombin. Activation involves cleavage at residue 37 by thrombin, releasing an activation peptide. A common polymorphism (valine to leucine variant at residue 34, V34L), located in the activation peptide, has been associated with increased activation rates and paradoxically a protective effect in cardiovascular disease. There is, currently, no data available on the effects of V34L from in vivo models of thrombosis. We examined the effect of FXIII V34L on clot formation and cross-linking in vivo. Approach and Results-We generated a panel of full-length recombinant human FXIII-A2 variants with amino acid substitutions in the activation peptide to investigate the effect of these variants on activation rate, and we used wild-type, V34L, and alanine to glycine variant at residue 33 variants to study the effects of varying FXIII activation rate on thrombus formation in a murine model of FeCl3 injury. FXIII activation assay showed that residues 29, 30, 33, and 34 play a critical role in thrombin interaction. Full-length recombinant human FXIII-A2 V34L has significant effects on clot formation, structure, and lysis in vitro, using turbidity assay. This variant influenced fibrin cross-linking but not size of the thrombus in vivo. Conclusions-Mutations in the activation peptide of full-length recombinant FXIII regulate activation rates by thrombin, and V34L influences in vivo thrombus formation by increased cross-linking of the clot.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 American Heart Association. This is an author produced version of a paper subsequently published in Arteriosclerosis, Thrombosis, and Vascular Biology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | factor XIII; fibrinogen; mutation; thrombin; venous thrombosis |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease |
Funding Information: | Funder Grant number BRITISH HEART FOUNDATION RG/13/3/30104 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 15 Feb 2017 12:39 |
Last Modified: | 03 Nov 2017 02:55 |
Published Version: | https://doi.org/10.1161/ATVBAHA.115.306695 |
Status: | Published |
Publisher: | Lippincott, Williams & Wilkins |
Refereed: | Yes |
Identification Number: | 10.1161/ATVBAHA.115.306695 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:111562 |