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Holder, BS, Jones, T, Sancho Shimizu, V et al. (5 more authors) (2016) Macrophage exosomes induce placental inflammatory cytokines: a novel mode of maternal-placental messaging. Traffic, 17 (2). pp. 168-178. ISSN 1398-9219
Abstract
During pregnancy, the placenta forms the interface between mother and fetus. Highly controlled regulation of trans-placental trafficking is therefore essential for the healthy development of the growing fetus. Extracellular vesicle-mediated transfer of protein and nucleic acids from the human placenta into the maternal circulation is well documented; the possibility that this trafficking is bi-directional has not yet been explored but could affect placental function and impact on the fetus. We hypothesized that the ability of the placenta to respond to maternal inflammatory signals is mediated by the interaction of maternal immune cell exosomes with placental trophoblast. Utilising the BeWo cell line and whole placental explants, we demonstrated that the human placenta internalizes macrophage-derived exosomes in a time- and dose-dependent manner. This uptake was via clathrin-dependent endocytosis. Furthermore, macrophage exosomes induced production of proinflammatory cytokines by the placenta. Taken together, our data demonstrates that exosomes are actively transported into the human placenta and that exosomes from activated immune cells modulate placental cytokine production. This represents a novel mechanism by which immune cells can signal to the placental unit, potentially facilitating responses to maternal inflammation and infection, and thereby preventing harm to the fetus.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 The Authors. Traffic published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | cytokines; endocytosis; exosomes; extracellular vesicles; immunology; macrophage; microvesicles; placenta; pregnancy; reproductive; trophoblast |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Genetics, Health and Therapeutics (LIGHT) > Division of Reproduction & Early Development Research (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 19 Jan 2017 11:23 |
Last Modified: | 19 Jan 2017 11:23 |
Published Version: | http://dx.doi.org/10.1111/tra.12352 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/tra.12352 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:110710 |
Available Versions of this Item
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Macrophage exosomes induce placental inflammatory cytokines: a novel mode of maternal-placental messaging. (deposited 29 Apr 2016 10:16)
- Macrophage exosomes induce placental inflammatory cytokines: a novel mode of maternal-placental messaging. (deposited 19 Jan 2017 11:23) [Currently Displayed]