Damjanov, I. and Andrews, P.W. orcid.org/0000-0001-7215-4410 (2016) Teratomas produced from human pluripotent stem cells xenografted into immunodeficient mice - A histopathology atlas. International Journal of Developmental Biology, 60 (10-12). pp. 337-419. ISSN 0214-6282
Abstract
This atlas illustrates the microscopic features of tumors produced from human pluripotent stem cells (hPSCs) xenografted into immunosuppressed mice, according to the generally accepted protocols for performing this teratoma assay of stem cell pluripotency. Microphotographs depict various hematoxylin and eosin (H&E) stained tissues derived from all three embryonic germ layers (ectoderm, mesoderm and endoderm). The appearance of persistent hPSC in teratomas is also described with special emphasis on the morphogenesis of embryoid bodies and yolk sac components surrounding them. The use of immunohistochemistry for analyzing hPSC-derived teratomas is also illustrated.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 UPV/EHU Press (Bilbao, Spain) and Creative Commons CC-BY. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/), which permits you to Share (copy and redistribute the material in any medium or format) and Adapt (remix, transform, and build upon the material for any purpose, even commercially), providing you give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. |
Keywords: | human pluripotent stem cells; ectoderm; mesoderm; endoderm; yolk sac |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 18 Jan 2017 15:14 |
Last Modified: | 18 Jan 2017 15:21 |
Published Version: | http://doi.org/10.1387/ijdb.160274id |
Status: | Published |
Publisher: | University of the Basque Country Press |
Refereed: | Yes |
Identification Number: | 10.1387/ijdb.160274id |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:110669 |