Alves da Silva, M, Lenton, S, Hughes, M et al. (2 more authors) (2017) Assessing the potential of folded globular polyproteins as hydrogel building blocks. Biomacromolecules, 18 (2). pp. 636-646. ISSN 1525-7797
Abstract
The native states of proteins generally have stable well-defined folded structures endowing these biomolecules with specific functionality and molecular recognition abilities. Here we explore the potential of using folded globular polyproteins as building blocks for hydrogels. Photo-chemically crosslinked hydrogels were produced from polyproteins containing either five domains of I27 ((I27)5), protein L ((pL)5) or a 1:1 blend of these proteins. SAXS analysis showed that (I27)5 exists as a single rod-like structure, while (pL)5 shows signatures of self-aggregation in solution. SANS measurements showed that both polyprotein hydrogels have a similar nanoscopic structure, with protein L hydrogels being formed from smaller and more compact clusters. The polyprotein hydrogels showed small energy dissipation in a load/unload cycle, which significantly increased when the hydrogels were formed in the unfolded state. This study demonstrates the use of folded proteins as building blocks in hydrogels, and highlights the potential versatility that can be offered in tuning the mechanical, structural and functional properties of polyproteins.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 American Chemical Society. This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Biomacromolecules, copyright © American Chemical Society after peer review. To access the final edited and published work see https://doi.org/10.1021/acs.biomac.6b01877 |
Keywords: | SANS; rheology; I27; protein L; atomic force spectroscopy; biomaterials |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Structural Molecular Biology 2 (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics |
Funding Information: | Funder Grant number EU - European Union 258259 EPSRC EP/H049479/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 12 Jan 2017 11:38 |
Last Modified: | 22 Dec 2017 01:38 |
Published Version: | https://doi.org/10.1021/acs.biomac.6b01877 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/acs.biomac.6b01877 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:110464 |