Howlett, R.M., Davey, M.P. and Kelly, D.J. orcid.org/0000-0002-0770-6845 (2016) Metabolomic Analysis of Campylobacter jejuni by Direct-Injection Electrospray Ionization Mass Spectrometry. In: Butcher, James and Stintzi, Alain, (eds.) Campylobacter jejuni. Methods in Molecular Biology, 1512 (1512). Springer , New York , pp. 189-197.
Abstract
Direct-injection mass spectrometry (DIMS) is a means of rapidly obtaining metabolomic phenotype data in both prokaryotes and eukaryotes. Given our generally poor understanding of Campylobacter metabolism, the high-throughput and relatively simple sample preparation of DIMS has made this an attractive technique for metabolism-related studies and hypothesis generation, especially when attempting to analyze metabolic mutants with no clear phenotype. Here we describe a metabolomic fingerprinting approach with sampling and extraction methodologies optimized for direct-injection electrospray ionization mass spectrometry (ESI-MS), which we have used as a means of comparing wild-type and isogenic mutant strains of C. jejuni with various metabolic blocks.
Metadata
Item Type: | Book Section |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © 2016 Springer Science+Business Media New York. This is an author produced version of a paper subsequently published in Methods in Molecular Biology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | DIMS; Direct injection; Electrospray ionization mass spectrometry; Fingerprinting; Metabolite extraction; Metabolome; Metabolomics |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 25 May 2017 13:45 |
Last Modified: | 25 Nov 2017 01:38 |
Published Version: | http://doi.org/10.1007/978-1-4939-6536-6_16 |
Status: | Published |
Publisher: | Springer |
Series Name: | Methods in Molecular Biology |
Refereed: | Yes |
Identification Number: | 10.1007/978-1-4939-6536-6_16 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:110412 |