Tellez-Gabriel, M., Charrier, C., Brounais-Le Royer, B. et al. (4 more authors) (2017) Analysis of gap junctional intercellular communications using a dielectrophoresis-based microchip. European Journal of Cell Biology, 96 (2). pp. 110-118. ISSN 0171-9335
Abstract
Gap junctions are transmembrane structures that directly connect the cytoplasm of adjacent cells, making intercellular communications possible. It has been shown that the behaviour of several tumours – such as bone tumours – is related to gap junction intercellular communications (GJIC). Several methodologies are available for studying GJIC, based on measuring different parameters that are useful for multiple applications, such as the study of carcinogenesis for example. These methods nevertheless have several limitations. The present manuscript describes the setting up of a DEP-based lab-on-a-chip platform for the real-time study of Gap Junctional Intercellular Communication between osteosarcoma cells and the main cells accessible to their microenvironment. We conclude that using the DEParray technology for the GJIC assessment has several advantages comparing to current techniques. This methodology is less harmful for cells integrity; cells can be recovered after interaction to make further molecular analysis; it is possible to study GJIC in real time; we can promote cell interactions using up to five different populations. The setting up of this new methodology overcomes several difficulties to perform experiments for solving questions about GJIC process that we are not able to do with current technics.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Elsevier. This is an author produced version of a paper subsequently published in European Journal of Cell Biology. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/) |
Keywords: | Gap junctional intercellular communication; Dielectrophoresis device; Bone microenvironment |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) |
Funding Information: | Funder Grant number BONE CANCER RESEARCH TRUST BCRT 4516 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 17 Jan 2017 15:16 |
Last Modified: | 07 Jul 2023 16:13 |
Published Version: | https://doi.org/10.1016/j.ejcb.2017.01.003 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.ejcb.2017.01.003 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:110295 |
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