Butler, James Andrew, Cosgrove, Jason, Alden, Kieran James et al. (2 more authors) (2016) Model-driven Experimentation: A new approach to understand mechanisms of tertiary lymphoid tissue formation, function and therapeutic resolution. Frontiers in immunology. pp. 1-23. ISSN 1664-3224
Abstract
The molecular and cellular processes driving the formation of secondary lymphoid tissues have been extensively studied using a combination of mouse knockouts, lineage specific reporter mice, gene expression analysis, immunohistochemistry and flow cytometry. However, the mechanisms driving the formation and function of tertiary lymphoid tissue (TLT) experimental techniques have proven to be more enigmatic and controversial due to differences between experimental models and human disease pathology. Systems-based approaches including data-driven biological network analysis (Gene Interaction Network, Metabolic Pathway Network, Cell-Cell signalling & cascade networks) and mechanistic modelling afford a novel perspective from which to understand TLT formation and identify mechanisms that may lead to the resolution of tissue pathology. In this perspective, we make the case for applying model-driven experimentation using two case studies which combined simulations with experiments to identify mechanisms driving lymphoid tissue formation and function, and then discuss potential applications of this experimental paradigm to identify novel therapeutic targets for TLT pathology.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Butler, Cosgrove, Alden, Timmis and Coles. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Electronic Engineering (York) The University of York > Faculty of Sciences (York) > Hull York Medical School (York) The University of York > Faculty of Sciences (York) > Centre for Immunology and Infection (CII) (York) |
Depositing User: | Pure (York) |
Date Deposited: | 21 Dec 2016 14:55 |
Last Modified: | 26 Nov 2024 00:33 |
Published Version: | https://doi.org/10.3389/fimmu.2016.00658 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.3389/fimmu.2016.00658 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:109823 |
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