Salman Qureshi, O., Zeb, A., Akram, M. et al. (8 more authors) (2016) Enhanced acute anti-inflammatory effects of CORM-2-loaded nanoparticles via sustained carbon monoxide delivery. European Journal of Pharmaceutics and Biopharmaceutics, 108. pp. 187-195. ISSN 0939-6411
Abstract
The aim of this study was to enhance the anti-inflammatory effects of carbon monoxide (CO) via sustained release of CO from carbon monoxide-releasing molecule-2-loaded lipid nanoparticles (CORM-2-NPs). CORM-2-NPs were prepared by hot high pressure homogenization method using trilaurin as a solid lipid core and Tween 20/Span 20/Myrj S40 as surfactants mixture. The physicochemical properties of CORM-2-NPs were characterized and CO release from CORM-2-NPs was assessed by myoglobin assay. In vitro anti-inflammatory effects were evaluated by nitric oxide assay in lipopolysaccharide-stimulated RAW 264.7 macrophages. In vivo anti-inflammatory activity was investigated by measuring paw volumes and histological examination in carrageenan-induced rat paw edema. Spherical CORM-2-NPs were around 100 nm with narrow particle size distribution. The sustained CO release from CORM-2-NPs was observed and the half-life of CO release increased upto 10 times compared with CORM-2 solution. CORM-2-NPs showed enhanced in vitro anti-inflammatory effects by inhibition of nitric oxide production. Edema volume in rat paw was significantly reduced after treatment with CORM-2-NPs. Taken together, CORM-2-NPs have a great potential for CO therapeutics against inflammation via sustained release of CO.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Elsevier |
Keywords: | Anti-inflammatory effect; CORM-2; Carbon monoxide; Lipid nanoparticles; Sodium dithionite (PubChem CID: 24489); Sustained release; Tricarbonyldichlororuthenium(II) dimer (PubChem CID: 10951331); Trilaurin (PubChem CID: 10851); Tween 20 (PubChem CID: 443314) |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Neuroscience (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 14 Dec 2016 10:28 |
Last Modified: | 14 Dec 2016 10:36 |
Published Version: | http://doi.org/10.1016/j.ejpb.2016.09.008 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.ejpb.2016.09.008 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:109294 |