West, S. and Proudfoot, N. J. (2008) Human Pcf11 enhances degradation of RNA polymerase II-associated nascent RNA and transcriptional termination. Nucleic Acids Research, 36 (3). pp. 905-914. ISSN 0305-1048
Abstract
The poly(A) (pA) signal possesses a dual function in 3′ end processing of pre-mRNA and in transcriptional termination of RNA polymerase II (Pol II) for most eukaryotic protein-coding genes. A key protein factor in yeast and Drosophila Pol II transcriptional termination is the 3′-end processing factor, Pcf11. In vitro studies suggest that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Moreover, several mutant alleles of yeast Pcf11 effect termination in vivo. However, functions of human Pcf11 (hPcf11) in Pol II termination have not been explored. Here we show that depletion of hPcf11 from HeLa cells reduces termination efficiency. Furthermore, we provide evidence that hPcf11 is required for the efficient degradation of the 3′ product of pA site cleavage. Finally, we show that these functions of hPcf11 require an intact pA signal.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | �© 2007 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | pulmonary arteriogram; pulmonary artery; transcription; genetic antimicrobial; chemoprophylaxis; dna; dna polymerase ii ;dna-directed rna polymerase; drosophila; genes; hela cells; panama; paraguay; pennsylvania; physician assistant; plasminogen activator; reverse transcriptase polymerase chain reaction; rna polymerase ii; heterogeneous nuclear rna; yeasts; polyarthritis; rna; cytokinesis; catabolism |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 05 Dec 2016 15:40 |
Last Modified: | 05 Dec 2016 15:41 |
Published Version: | http://dx.doi.org/10.1093/nar/gkm1112 |
Status: | Published |
Publisher: | Oxford University Press (OUP): Policy C - Option B |
Refereed: | Yes |
Identification Number: | 10.1093/nar/gkm1112 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108674 |