Morra, R, Fessl, T orcid.org/0000-0001-6969-4870, Wang, Y et al. (2 more authors) (2016) Biophysical characterization of chromatin remodeling protein CHD4. In: Leake, MC, (ed.) Chromosome Architecture: Methods and Protocols. Methods in Molecular Biology (1431). Springer , New York, USA , pp. 175-193. ISBN 978-1-4939-3629-8
Abstract
Chromatin-remodeling ATPases modulate histones–DNA interactions within nucleosomes and regulate transcription. At the heart of remodeling, ATPase is a helicase-like motor flanked by a variety of conserved targeting domains. CHD4 is the core subunit of the nucleosome remodeling and deacetylase complex NuRD and harbors tandem plant homeo finger (tPHD) and chromo (tCHD) domains. We describe a multifaceted approach to link the domain structure with function, using quantitative assays for DNA and histone binding, ATPase activity, shape reconstruction from solution scattering data, and single molecule translocation assays. These approaches are complementary to high-resolution structure determination.
Metadata
Item Type: | Book Section |
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Authors/Creators: |
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Editors: |
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Keywords: | Nucleosome; ATPase; Surface plasmon resonance; SAXS; TIRF; FRET |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 28 Nov 2016 09:30 |
Last Modified: | 28 Nov 2016 09:30 |
Published Version: | https://doi.org/10.1007/978-1-4939-3631-1_14 |
Status: | Published |
Publisher: | Springer |
Series Name: | Methods in Molecular Biology |
Identification Number: | 10.1007/978-1-4939-3631-1_14 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108554 |