Kweider, N, Huppertz, B, Rath, W et al. (8 more authors) (2017) The effects of Nrf2 deletion on placental morphology and exchange capacity in the mouse. Journal of Maternal-Fetal and Neonatal Medicine, 30 (17). pp. 2068-2073. ISSN 1476-7058
Abstract
Objectives: Intrauterine growth restriction (IUGR) is defined as a pathological decreased fetal growth. Oxidative stress has been connected to the restriction in the fetal growth. The transcription factor nuclear factor-erythroid 2-related factor 2 (Nrf2) is a potent activator of the cellular antioxidant response. The effect Nrf2 on fetal–placental development has not yet been sufficiently investigated. Here, we evaluated the placental and fetal growth in Nrf2 knockout (Nrf2-KO) and Nrf2-wild type mice (Nrf2-WT) throughout pregnancy. Methods: Heterozygote Nrf2 (Nrf2+/−) mice were paired to get Nrf2-KO and Nrf2-WT in the litters. Placentae and embryos from both genotypes were collected and weighed on days 13.5, 15.5 and 18.5 post coitum. The absolute volumes of the labyrinth zone and the total volume of the placenta were determined using the Cavalieri principle. Results: On E 18.5 the fetal weight in Nrf2-KO was significantly reduced versus Nrf2-WT indicating a decrease in placental efficiency. A significant reduction in both total and labyrinth-volume in the placenta of Nrf2-KO mice was observed. Conclusion: This data points out the necessity of functional Nrf2 for fetal and placental growth. A deficiency in Nrf2 signaling may negatively affect nutrient transfer capacity which is then no longer able to meet fetal growth demands.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Nrf2, oxidative stress, IUGR, placenta, placental insufficiency |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 28 Nov 2016 12:45 |
Last Modified: | 24 Aug 2017 13:57 |
Published Version: | https://doi.org/10.1080/14767058.2016.1236251 |
Status: | Published |
Publisher: | Taylor & Francis |
Identification Number: | 10.1080/14767058.2016.1236251 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108531 |