Smythe, E., Carter, L. L. and Schmid, S. L. (1992) Cytosol- and clathrin-dependent stimulation of endocytosis in vitro by purified adaptors. Journal of Cell Biology, 119 (5). pp. 1163-1171. ISSN 0021-9525
Abstract
Using stage-specific assays for receptor-mediated endocytosis of transferrin (Tfn) into perforated A431 cells we show that purified adaptors stimulate coated pit assembly and ligand sequestration into deeply invaginated coated pits. Late events in endocytosis involving membrane fission and coated vesicle budding which lead to the internalization of Tfn are unaffected. AP2, plasma membrane adaptors, are active at physiological concentrations, whereas AP1, Golgi adaptors, are inactive. Adaptor-dependent stimulation of Tfn sequestration requires cytosolic clathrin, but is unaffected by clathrin purified from coated vesicles suggesting that soluble and assembled clathrin pools are functionally distinct. In addition to adaptors and cytosolic clathrin other, as yet unidentified, cytosolic factors are also required for efficient coated pit invagination. These results provide new insight into the mechanisms and regulation of coated pit assembly and invagination.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 1992 Rockefeller University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial Share-Alike Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. You may not use the material for commercial purposes. |
Keywords: | Adaptor Protein Complex 1; Adaptor Protein Complex 2; Adaptor Protein Complex alpha Subunits; Adaptor Proteins, Vesicular Transport; Biological Transport; Cell Compartmentation; Cell Membrane Permeability; Cells, Cultured; Clathrin; Coated Pits, Cell-Membrane; Cytosol; Endocytosis; Humans; Proteins; Stimulation, Chemical; Transferrin |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 21 Dec 2016 14:54 |
Last Modified: | 21 Dec 2016 15:00 |
Published Version: | http://dx.doi.org/10.1083/jcb.119.5.1163 |
Status: | Published |
Publisher: | Rockefeller University Press |
Refereed: | Yes |
Identification Number: | 10.1083/jcb.119.5.1163 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108306 |