Nassar, M. A., Baker, M. D., Levato, A. et al. (4 more authors) (2006) Nerve injury induces robust allodynia and ectopic discharges in Nav1.3 null mutant mice. Molecular Pain, 2. 33. ISSN 1744-8069
Abstract
Changes in sodium channel activity and neuronal hyperexcitability contribute to neuropathic pain, a major clinical problem. There is strong evidence that the re-expression of the embryonic voltage-gated sodium channel subunit Nav1.3 underlies neuronal hyperexcitability and neuropathic pain. Here we show that acute and inflammatory pain behaviour is unchanged in global Nav1.3 mutant mice. Surprisingly, neuropathic pain also developed normally in the Nav1.3 mutant mouse. To rule out any genetic compensation mechanisms that may have masked the phenotype, we investigated neuropathic pain in two conditional Nav1.3 mutant mouse lines. We used Nav1.8-Cre mice to delete Nav1.3 in nociceptors at E14 and NFH-Cre mice to delete Nav1.3 throughout the nervous system postnatally. Again normal levels of neuropathic pain developed after nerve injury in both lines. Furthermore, ectopic discharges from damaged nerves were unaffected by the absence of Nav1.3 in global knock-out mice. Our data demonstrate that Nav1.3 is neither necessary nor sufficient for the development of nerve-injury related pain.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2006 Nassar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Amino Acid Sequence; Animals; Base Sequence; Exons; Female; Gene Deletion; Inflammation; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Data; NAV1.3 Voltage-Gated Sodium Channel; Neurons, Afferent; Pain; Sequence Analysis, DNA; Sodium Channels |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 21 Dec 2016 15:46 |
Last Modified: | 21 Dec 2016 15:51 |
Published Version: | http://journals.sagepub.com/doi/full/10.1186/1744-... |
Status: | Published |
Publisher: | SAGE Publications (UK and US): Open Access Titles |
Refereed: | Yes |
Identification Number: | 10.1186/1744-8069-2-33 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108286 |