Cambray-Deakin, M. A. and Burgoyne, R. D. (1987) Posttranslational modifications of alpha-tubulin: acetylated and detyrosinated forms in axons of rat cerebellum. Journal of Cell Biology, 104 (6). pp. 1569-1574. ISSN 0021-9525
Abstract
The distribution of acetylated alpha-tubulin in rat cerebellum was examined and compared with that of total alpha-tubulin and tyrosinated alpha-tubulin. From immunoperoxidase-stained vibratome sections of rat cerebellum it was found that acetylated alpha-tubulin, detectable with monoclonal 6-11B-1, was preferentially enriched in axons compared with dendrites. Parallel fiber axons, in particular, were labeled with 6-11B-1 yet unstained by an antibody recognizing tyrosinated alpha-tubulin, indicating that parallel fibers contain alpha-tubulin that is acetylated and detyrosinated. Axonal microtubules are known to be highly stable and the distribution of acetylated alpha-tubulin in other classes of stable microtubules suggests that acetylation and possibly detyrosination may play a role in the maintenance of stable populations of microtubules.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 1987 Rockefeller University Press. CC BY-NC-SA. his is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share-alike License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Acetylation; Animals; Axons; Brain Chemistry; Cells, Cultured; Cerebellum; Dendrites; Immunoenzyme Techniques; Neuroglia; Protein Processing, Post-Translational; Purkinje Cells; Rats; Tubulin; Tyrosine |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 07 Dec 2016 16:32 |
Last Modified: | 07 Dec 2016 16:36 |
Published Version: | http://dx.doi.org/10.1083/jcb.104.6.1569 |
Status: | Published |
Publisher: | Rockefeller University Press |
Refereed: | Yes |
Identification Number: | 10.1083/jcb.104.6.1569 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108265 |