Aghamohammadzadeh, S. and Ayscough, K.R. orcid.org/0000-0003-3716-4490 (2009) Differential requirements for actin during yeast and mammalian endocytosis. Nature Cell Biology , 11 (8). pp. 1039-1042. ISSN 1465-7392
Abstract
Key features of clathrin-mediated endocytosis have been conserved across evolution. However, endocytosis in Saccharomyces cerevisiae is completely dependent on a functional actin cytoskeleton, whereas actin appears to be less critical in mammalian cell endocytosis. We reveal that the fundamental requirement for actin in the early stages of yeast endocytosis is to provide a strong framework to support the force generation needed to direct the invaginating plasma membrane into the cell against turgor pressure. By providing osmotic support, pressure differences across the plasma membrane were removed and this reduced the requirement for actin-bundling proteins in normal endocytosis. Conversely, increased turgor pressure in specific yeast mutants correlated with a decreased rate of endocytic patch invagination.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2009 Macmillan Publishers Limited. This is an author produced version of a paper subsequently published in Nature Cell Biology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | PPZ Protein Phosphatases; Cell Polarity; Cytoskeleton; Invagination; Recruitment; Pathway |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 24 Nov 2016 13:56 |
Last Modified: | 27 Mar 2018 03:37 |
Published Version: | https://doi.org/10.1038/ncb1918 |
Status: | Published |
Publisher: | Nature Publishing Group |
Refereed: | Yes |
Identification Number: | 10.1038/ncb1918 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108257 |