Meireles, A.M., Fisher, K.H. orcid.org/0000-0002-2751-156X, Colombié, N. et al. (2 more authors) (2009) Wac: a new Augmin subunit required for chromosome alignment but not for acentrosomal microtubule assembly in female meiosis. The Journal of Cell Biology (JCB), 184 (6). pp. 777-784. ISSN 0021-9525
Abstract
The bipolar spindle forms without centrosomes naturally in female meiosis and by experimental manipulation in mitosis. Augmin is a recently discovered protein complex required for centrosome-independent microtubule generation within the spindle in Drosophila melanogaster cultured cells. Five subunits of Augmin have been identified so far, but neither their organization within the complex nor their role in developing organisms is known. In this study, we report a new Augmin subunit, wee Augmin component (Wac). Wac directly interacts with another Augmin subunit, Dgt2, via its coiled-coil domain. Wac depletion in cultured cells, especially without functional centrosomes, causes severe defects in spindle assembly. We found that a wac deletion mutant is viable but female sterile and shows only a mild impact on somatic mitosis. Unexpectedly, mutant female meiosis showed robust microtubule assembly of the acentrosomal spindle but frequent chromosome misalignment. For the first time, this study establishes the role of an Augmin subunit in developing organisms and provides an insight into the architecture of the complex.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2009 Meireles et al. This article is distributed under the terms of an Attribution– Noncommercial–Share Alike–No Mirror Sites license for the fi rst six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
Keywords: | Amino Acid Sequence; Animals; Carrier Proteins; Cell Proliferation; Cell Survival; Cells, Cultured; Centrosome; Chromosome Segregation; Drosophila Proteins; Drosophila melanogaster; Female; Fertility; Gene Expression Regulation, Developmental; Kinetochores; Larva; Male; Meiosis; Microtubule-Associated Proteins; Microtubules; Mitosis; Molecular Sequence Data; Multiprotein Complexes; RNA Interference; Sequence Deletion |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 09 Jan 2017 16:42 |
Last Modified: | 09 Jan 2017 16:50 |
Published Version: | http://doi.org/10.1083/jcb.200811102 |
Status: | Published |
Publisher: | Rockefeller University Press |
Refereed: | Yes |
Identification Number: | 10.1083/jcb.200811102 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108199 |