Xu, L., Sedelnikova, S.E., Baker, P.J. orcid.org/0000-0003-1995-5643 et al. (1 more author) (2006) Cloning, purification and preliminary crystallographic analysis of a conserved hypothetical protein, SA0961 (YlaN), from Staphylococcus aureus. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, F62. pp. 778-780. ISSN 1744-3091
Abstract
SA0961 is an unknown hypothetical protein from Staphylococcus aureus that can be identified in the Firmicutes division of Gram-positive bacteria. The gene for the homologue of SA0961 in Bacillus subtilis, ylaN, has been shown to be essential for cell survival, thus identifying the protein encoded by this gene as a potential target for the development of novel antibiotics. SA0961 was cloned and the protein was overexpressed in Escherichia coli, purified and subsequently crystallized. Crystals of selenomethionine-labelled SA0961 diffract to beyond 2.4 Å resolution and belong to the monoclinic space group P21, with unit-cell parameters a = 31.5, b = 42.7, c = 62.7 Å, [beta] = 92.4° and two molecules in the asymmetric unit. A full structure determination is under way to provide insights into the function of this protein.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2006 International Union of Crystallography. Article available Open Access. Article available under the terms of the CC-BY licence. |
Keywords: | SA0961; YlaN; Staphylococcus aureus |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 06 Jan 2017 11:29 |
Last Modified: | 06 Jan 2017 11:29 |
Published Version: | https://doi.org/10.1107/S1744309106027400 |
Status: | Published |
Publisher: | International Union of Crystallography |
Refereed: | Yes |
Identification Number: | 10.1107/S1744309106027400 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:108134 |