Sanders, C. M., Kovalevskiy, O. V., Sizov, D. et al. (3 more authors) (2007) Papillomavirus E1 helicase assembly maintains an asymmetric state in the absence of DNA and nucleotide cofactors. Nucleic Acids Research , 35 (19). pp. 6451-6457. ISSN 0305-1048
Abstract
Concerted, stochastic and sequential mechanisms of action have been proposed for different hexameric AAA+ molecular motors. Here we report the crystal structure of the E1 helicase from bovine papillomavirus, where asymmetric assembly is for the first time observed in the absence of nucleotide cofactors and DNA. Surprisingly, the ATP-binding sites adopt specific conformations linked to positional changes in the DNA-binding hairpins, which follow a wave-like trajectory, as observed previously in the E1/DNA/ADP complex. The protein's assembly thus maintains such an asymmetric state in the absence of DNA and nucleotide cofactors, allowing consideration of the E1 helicase action as the propagation of a conformational wave around the protein ring. The data imply that the wave's propagation within the AAA+ domains is not necessarily coupled with a strictly sequential hydrolysis of ATP. Since a single ATP hydrolysis event would affect the whole hexamer, such events may simply serve to rectify the direction of the wave's motion.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2007 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | REPLICATIVE HEXAMERIC HELICASE; MOLECULAR-GRAPHICS; CRYSTAL-STRUCTURE; ATP HYDROLYSIS; TRANSLOCATION; MECHANISMS; E1 |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 21 Nov 2016 14:00 |
Last Modified: | 21 Nov 2016 14:05 |
Published Version: | http://dx.doi.org/10.1093/nar/gkm705 |
Status: | Published |
Publisher: | Oxford University Press (OUP): Policy C - Option B |
Refereed: | Yes |
Identification Number: | 10.1093/nar/gkm705 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:107960 |