Gillespie, A.M., Rodgers, S., Wilson, A.P. et al. (4 more authors) (1998) MAGE, BAGE and GAGE: tumour antigen expression in benign and malignant ovarian tissue. British Journal of Cancer, 78 (6). pp. 816-821. ISSN 0007-0920
Abstract
To determine if ovarian cancer patients would be suitable for MAGE-peptide vaccine-based immunotherapy, the frequency of expression of the MAGE-1-4 genes in ovarian tumours was assessed using reverse transcription polymerase chain reaction (RT-PCR) and product verification with digoxigenin-labelled oligonucleotide probes specific for each MAGEgene. In addition, the frequency of expression of more recently discovered tumour antigens (BAGE, GAGE -1, -2 and GAGE -3, -6) was established using RT-PCR and ethidium bromide staining. In this study 1/16 normal ovarian tissue specimens and 11/25 benign lesions expressed MAGE-1. In non-malignant tissue there was preferential expression of MAGE-1 in premenopausal women. A total of 15/27 malignant specimens expressed MAGE-1, including 10/14 serous cystadenocarcinomas. Expression of other tumour antigens was infrequent. The finding of MAGE-1 expression in both benign and malignant tissue questions previous assumptions regarding the role of MAGEgenes in carcinogenesis. In addition, preferential MAGE-1 gene expression in non-malignant premenopausal tissue suggests that the MAGE genes may be involved in cellular proliferation as opposed to carcinogenesis or possibly that MAGE gene expression is under cyclical hormonal control. Finally, this study indicates that serous cystadenocarcinomas may be suitable tumours for MAGE-1 peptide immunotherapy.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 1998 Cancer Research Campaign. Twelve months after publication in an issue of BJC, all content is made freely available to readers via PubMed Central and the BJC website (www.bjcancer.com) under the terms of BJC’s attribution, non commercial, share alike licence (CC BY NC SA) |
Keywords: | MAGE; BAGE; GAGE; ovarian; tumour antigen; cancer immunotherapy |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 16 Dec 2016 10:25 |
Last Modified: | 16 Dec 2016 11:42 |
Published Version: | https://doi.org/10.1038/bjc.1998.585 |
Status: | Published |
Publisher: | Cancer Research UK |
Refereed: | Yes |
Identification Number: | 10.1038/bjc.1998.585 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:107814 |
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