Andersen, MCF, Boos, I, Marcus, SE et al. (5 more authors) (2016) Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-d-galactotetraose. Carbohydrate Research, 436. pp. 36-40. ISSN 0008-6215
Abstract
Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies for localization and visualization of glycans, using e.g. Immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mab LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogs of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016, Elsevier. This is an author produced version of a paper published in Carbohydrate Research. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Nov 2016 16:18 |
Last Modified: | 31 Oct 2017 22:19 |
Published Version: | https://doi.org/10.1016/j.carres.2016.10.012 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.carres.2016.10.012 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:107151 |