Rawlings, A.E., Bramble, J.P., Hounslow, A.M. et al. (4 more authors) (2016) Ferrous Iron Binding Key to Mms6 Magnetite Biomineralisation: A Mechanistic Study to Understand Magnetite Formation Using pH Titration and NMR Spectroscopy. Chemistry - A European Journal, 22 (23). pp. 7885-7894. ISSN 0947-6539
Abstract
Formation of magnetite nanocrystals by magn eto-tactic bacteria is controlled by specificproteins whichregu-late the particles’ nucleation and growth.One such proteinis Mms6. This small, amphiph ilic protein can self-assembleand bind ferric ions to aid in magnetite formation. To under-stand the role of Mms6 duringinvitro iron oxide precipita-tion we have performed in situ pH titrations. We find Mms6has little effect duringferric salt precipitation, but exertsgreatest influence during the incorporation of ferrous ionsand conversion of this salt to mixed-valence iron mineral s,suggesting Mms6 has ahitherto unrecorded ferrous iron in-teracting property which promotes the formation of mag-netiteinferrous-rich solutions.Weshow ferrous binding tothe DEEVE motif within the C-term inal region of Mms6 byNMR spectroscopy,and model these binding events usingmolecular simulations. We conclude that Mms6 functions asamagnetite nucleating protein under cond itions where fer-rous ions predominate.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | biomimetic synthesis; magnetite; magnetotactic bacteria; mineralization; NMR spectroscopy |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Funding Information: | Funder Grant number BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL (BBSRC) BB/H005412/2 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 02 Nov 2016 15:41 |
Last Modified: | 03 Apr 2019 12:31 |
Published Version: | http://doi.org/10.1002/chem.201600322 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/chem.201600322 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:106874 |
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