Bulgakova, N.A. orcid.org/0000-0002-3780-8164, Rentsch, M. and Knust, E. (2010) Antagonistic functions of two stardust isoforms in Drosophila photoreceptor cells. Molecular Biology of the Cell, 21 (22). pp. 3915-3925. ISSN 1059-1524
Abstract
Membrane-associated guanylate kinases (MAGUKs) are scaffolding proteins that organize supramolecular protein complexes, thereby partitioning the plasma membrane into spatially and functionally distinct subdomains. Their modular organization is ideally suited to organize protein complexes with cell type- or stage-specific composition, or both. Often more than one MAGUK isoform is expressed by one gene in the same cell, yet very little is known about their individual in vivo functions. Here, we show that two isoforms of Drosophila stardust, Sdt-H (formerly called Sdt-B2) and Sdt-D, which differ in their N terminus, are expressed in adult photoreceptors. Both isoforms associate with Crumbs and PATJ, constituents of the conserved Crumbs-Stardust complex. However, they form distinct complexes, localized at the stalk, a restricted region of the apical plasma membrane. Strikingly, Sdt-H and Sdt-D have antagonistic functions. While Sdt-H overexpression increases stalk membrane length and prevents light-dependent retinal degeneration, Sdt-D overexpression reduces stalk length and enhances light-dependent retinal degeneration. These results suggest that a fine-tuned balance of different Crumbs complexes regulates photoreceptor homeostasis.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2010 N. A. Bulgakova et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). |
Keywords: | Animals; Animals, Genetically Modified; Blotting, Western; Down-Regulation; Drosophila Proteins; Drosophila melanogaster; Eye; Eye Proteins; Gene Expression Regulation, Developmental; Guanylate Kinase; Immunoprecipitation; Membrane Proteins; Membrane Transport Proteins; Microscopy, Electron, Transmission; Mutation; Photoreceptor Cells, Invertebrate; Protein Binding; Protein Isoforms; Protein Kinase C; Reverse Transcriptase Polymerase Chain Reaction |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 04 Nov 2016 16:11 |
Last Modified: | 04 Nov 2016 16:11 |
Published Version: | https://doi.org/10.1091/mbc.E09-10-0917 |
Status: | Published |
Publisher: | American Society for Cell Biology |
Refereed: | Yes |
Identification Number: | 10.1091/mbc.E09-10-0917 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:106702 |