Bourkoula, A, Constantoudis, V, Kontziampasis, D orcid.org/0000-0002-6787-8892 et al. (4 more authors) (2016) Roughness threshold for cell attachment and proliferation on plasma micro-nanotextured polymeric surfaces: The case of primary human skin fibroblasts and mouse immortalized 3T3 fibroblasts. Journal of Physics D: Applied Physics, 49 (30). 304002. ISSN 0022-3727
Abstract
Poly(methyl methacrylate) surfaces have been micro-nanotextured in oxygen plasmas with increasing ion energy, leading to micro-nanotopography characterized by increased root mean square roughness, correlation length and fractal dimension. Primary human skin fibroblasts and mouse immortalized 3T3 fibroblasts were cultured on these surfaces and the number of adhering cells, their proliferation rate and morphology (cytoplasm and nucleus area) were evaluated as a function of roughness height, correlation length, and fractal dimension. A roughness threshold behavior was observed for both types of cells leading to dramatic cell number decrease above this threshold, which is almost similar for the two types of cells, despite their differences in size and stiffness. The results are discussed based on two theoretical models, which are reconciled and unified when the elastic moduli and the size of the cells are taken into account.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2016 IOP Publishing Ltd. This is an author-created, un-copyedited version of an article published in Journal of Physics D: Applied Physics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/0022-3727/49/30/304002. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | poly(methyl methacrylate) surfaces, cell adhesion, cell proliferation, oxygen plasma nanotexturing |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) > Institute for Particle Science and Engineering (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 31 Oct 2016 11:51 |
Last Modified: | 19 Jul 2017 06:00 |
Published Version: | https://doi.org/10.1088/0022-3727/49/30/304002 |
Status: | Published |
Publisher: | IOP Publishing |
Identification Number: | 10.1088/0022-3727/49/30/304002 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:106688 |