Salawu, A., Fernando, M., Hughes, D. et al. (6 more authors) (2016) Establishment and molecular characterisation of seven novel soft-tissue sarcoma cell lines. British Journal of Cancer. ISSN 0007-0920
Abstract
Background:Soft-tissue sarcomas (STS) are a diverse group of malignancies that remain a diagnostic and therapeutic challenge. Relatively few reliable cell lines currently exist. Rapidly developing technology for genomic profiling with emerging insights into candidate functional (driver) aberrations raises the need for more models for in vitro functional validation of molecular targets.Methods:Primary cell culture was performed on STS tumours utilising a differential attachment approach. Cell lines were characterised by morphology, immunocytochemistry, proliferation assays, short tandem repeat (STR) and microarray-based genomic copy number profiling.Results:Of 47 STS cases of various subtypes, half formed adherent monolayers. Seven formed self-immortalised cell lines, including three undifferentiated pleomorphic sarcomas, two dedifferentiated liposarcomas (one of which had received radiotherapy), a leiomyosarcoma and a myxofibrosarcoma. Two morphologically distinct yet genetically identical variants were established in separate cultures for the latter two tumours. All cell lines demonstrated genomic and phenotypic features that not only confirm their malignant characteristics but also confirm retention of DNA copy number aberrations present in their parent tumours that likely include drivers.Conclusions:These primary cell lines are much-needed additions to the number of reliable cell lines of STS with complex genomics available for initial functional validation of candidate molecular targets.British Journal of Cancer advance online publication 25 August 2016; doi:10.1038/bjc.2016.259 www.bjcancer.com.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 Cancer Research UK. This is an author produced version of a paper subsequently published in British Journal of Cancer. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | sarcoma; primary cell line; in vitro models; soft-tissue sarcoma; array CGH; copy number profiles; short tandem repeat |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 17 Oct 2016 15:57 |
Last Modified: | 14 Apr 2017 02:42 |
Published Version: | http://dx.doi.org/10.1038/bjc.2016.259 |
Status: | Published |
Publisher: | Cancer Research UK |
Refereed: | Yes |
Identification Number: | 10.1038/bjc.2016.259 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:106050 |