Harrison, PL, Heath, GR, Johnson, BRG et al. (4 more authors) (2016) Phospholipid dependent mechanism of smp24, an α-helical antimicrobial peptide from scorpion venom. Biochimica et Biophysica Acta - Biomembranes, 1858 (11). pp. 2737-2744. ISSN 0005-2736
Abstract
Determining the mechanism of action of antimicrobial peptides (AMPs) is critical if they are to be developed into the clinical setting. In recent years high resolution techniques such as atomic force microscopy (AFM) have increasingly been utilised to determine AMP mechanism of action on planar lipid bilayers and live bacteria. Here we present the biophysical characterisation of a prototypical AMP from the venom of the North African scorpion Scorpio maurus palmatus termed Smp24. Smp24 is an amphipathic helical peptide containing 24 residues with a charge of + 3 and exhibits both antimicrobial and cytotoxic activity and we aim to elucidate the mechanism of action of this peptide on both membrane systems. Using AFM, quartz crystal microbalance-dissipation (QCM-D) and liposomal leakage assays the effect of Smp24 on prototypical synthetic prokaryotic (DOPG:DOPC) and eukaryotic (DOPE:DOPC) membranes has been determined. Our data points to a toroidal pore mechanism against the prokaryotic like membrane whilst the formation of hexagonal phase non-lamellar phase structures is seen in eukaryotic like membrane. Also, phase segregation is observed against the eukaryotic membrane and this study provides direct evidence of the same peptide having multiple mechanisms of action depending on the membrane lipid composition.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
Keywords: | Antimicrobial peptides; Membrane damage; Atomic force microscopy; Quartz crystal microbalance-dissipation |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics |
Depositing User: | Symplectic Publications |
Date Deposited: | 30 Sep 2016 08:32 |
Last Modified: | 05 Oct 2017 16:35 |
Published Version: | http://dx.doi.org/10.1016/j.bbamem.2016.07.018 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.bbamem.2016.07.018 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:105396 |