Clarke, EL and Treanor, D orcid.org/0000-0002-4579-484X (2017) Colour in Digital Pathology: A Review. Histopathology, 70 (2). pp. 153-163. ISSN 0309-0167
Abstract
Colour is central and integral to the practice of pathology; pathologists use coloured histochemical and immunohistochemical stains to identify structures and reach diagnoses. Given the magnitude of these diagnoses to patient management and outcome, it is imperative that pathologists are able to make accurate and reliable assertions. Over recent years, new technology has emerged to enable pathologists to carry out their diagnostic work digitaColour is central to the practice of pathology because of the use of coloured histochemical and immunohistochemical stains to visualize tissue features. Our reliance upon histochemical stains and light microscopy has evolved alongside a wide variation in slide colour, with little investigation into the implications of colour variation. However, the introduction of the digital microscope and whole-slide imaging has highlighted the need for further understanding and control of colour. This is because the digitization process itself introduces further colour variation which may affect diagnosis, and image analysis algorithms often use colour or intensity measures to detect or measure tissue features. The US Food and Drug Administration have released recent guidance stating the need to develop a method of controlling colour reproduction throughout the digitization process in whole-slide imaging for primary diagnostic use. This comprehensive review introduces applied basic colour physics and colour interpretation by the human visual system, before discussing the importance of colour in pathology. The process of colour calibration and its application to pathology are also included, as well as a summary of the current guidelines and recommendations regarding colour in digital pathology.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Colour in Digital Pathology: A Review, Clarke, E.L. and Treamor, D, Histopathology, which has been published in final form at https://doi.org/10.1111/his.13079. This article may be used for non-commercial purposes in accordance with the Wiley Terms and Conditions for Self-Archiving. |
Keywords: | Colour calibration; colour standardisation; digital pathology; whole slide imaging |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cancer and Pathology (LICAP) > Pathology & Tumour Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Sep 2016 08:53 |
Last Modified: | 13 Sep 2017 03:41 |
Published Version: | https://doi.org/10.1111/his.13079 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/his.13079 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:105136 |