Castangia, Roberto, Hudson, Sian, Robinson, Helen et al. (3 more authors) (2016) Fabrication and Application of Isotopically Labelled Gold Arrays for Multiplexed Peptide Analysis. Chembiochem. ISSN 1439-7633
Abstract
A new array-based technology for the simultaneous capture, chemical labelling and mass spectrometry analysis of peptides is presented. Isotopically labelled self-assembled monolayer (SAM) gold arrays are constructed and used simultaneously to capture and label a range of peptides. The array-immobilised, labelled peptides were released by MALDI ablation, analysed by MALDI mass spectrometry and readily identified as labelled peptides from their characteristic isotope pattern. This new solid-phase array platform has the advantage of minimal sample manipulation and is suitable for multiple analyses of single protein digests on a single MALDI target plate.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 19 Sep 2016 08:54 |
Last Modified: | 16 Oct 2024 13:16 |
Published Version: | https://doi.org/10.1002/cbic.201600347 |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1002/cbic.201600347 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:104884 |