Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA

Abstract

The first transition-metal complex-based two-photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging. In this initial study, ruthenium(II) luminophores are used as phosphorescent lifetime imaging microscopy (PLIM) probes for nuclear DNA in both live and fixed cells. The DNA-bound probes display characteristic emission lifetimes of more than 160 ns, while shorter-lived cytoplasmic emission is also observed. These timescales are orders of magnitude longer than conventional FLIM, leading to previously unattainable levels of sensitivity, and autofluorescence-free imaging.

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Item Type:	Article
Authors/Creators:	Baggaley, E. Gill, M.R. Green, N.H. https://orcid.org/0000-0001-5413-0642 Turton, D. Sazanovich, I.V. Botchway, S.W. Smythe, C. Haycock, J.W. Weinstein, J.A. Thomas, J.A.
Copyright, Publisher and Additional Information:	© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Dates:	Published: 19 March 2014 Published (online): 23 January 2014
Institution:	The University of Sheffield
Academic Units:	The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield)
Depositing User:	Symplectic Sheffield
Date Deposited:	08 Sep 2016 14:25
Last Modified:	08 Sep 2016 14:25
Published Version:	https://dx.doi.org/10.1002/ange.201309427
Status:	Published
Publisher:	Wiley
Refereed:	Yes
Identification Number:	10.1002/ange.201309427
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:104500

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Dinuclear Ruthenium(II) Complexes as Two-Photon, Time-Resolved Emission Microscopy Probes for Cellular DNA

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