Ricotti, L, Taccola, S, Bernardeschi, I et al. (3 more authors) (2011) Quantification of growth and differentiation of C2C12 skeletal muscle cells on PSS–PAH-based polyelectrolyte layer-by-layer nanofilms. Biomedical Materials, 6 (3). 031001. ISSN 1748-6041
Abstract
Polyelectrolyte layer-by-layer (LbL) nanofilms are interesting polymeric structures, built by alternating adsorption of positively and negatively charged polyelectrolytes. They consist of multilayer sheets with nanometric overall thickness, and they can be used as supports and surface coatings for in vitro and in vivo cell and tissue growth and regeneration. The present study focuses on nanofilms based on alternated layers of poly(sodium-4-sulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) fabricated using spin-assisted LbL assembly (SA-LbL). The fabrication process used to assemble polyelectrolyte nanofilms made of up to 60 bilayers is described, and the influence of different surface charges (i.e. changing the terminal layer) and of different film composition (e.g. varying PSS molecular weight) on cell behaviour is investigated. In particular, C2C12 skeletal muscle cells' viability, proliferation and differentiation on six different typologies of polyelectrolyte nanofilms are evaluated and quantified, giving a reference for skeletal muscle regeneration capabilities on such kind of structures.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2011 IOP Publishing Ltd. This is an author-created, un-copyedited version of an article published in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/1748-6041/6/3/031001 |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 May 2019 10:32 |
Last Modified: | 23 Jun 2023 22:13 |
Status: | Published |
Publisher: | IOP Publishing |
Identification Number: | 10.1088/1748-6041/6/3/031001 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:104499 |