Kirichek, O., Soper, A.K., Dzyuba, B. et al. (1 more author) (2016) Segregated water observed in a putative fish embryo cryopreservative. Royal Society Open Science, 3 (3). 150655. ISSN 2054-5703
Abstract
Development of new cryopreservation strategies has major potential in medicine and agriculture and is critical to the conservation of endangered species that currently cannot be preserved. A critical property of any potential cryopreservative solution is its ability to prevent cell-damaging ice formation during cooling and subsequent heating. This study focuses on the freezing behaviour of promising model cryoprotective solutions. We perform neutron scattering analysis, combined with computer modelling, of the water structure after quench cooling these solutions. It is found that water in this solution forms nano-clusters encapsulated by the surrounding matrix of cryoprotectant solute molecules. We posit that these small volumes inhibit ice formation, because water does not have space for the structural relaxation required to crystallize on the timescale of the cooling process.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, provided the original author and source are credited. |
Keywords: | common carp embryo; neutron scattering; cryopreservative; water structure |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > The Medical School (Sheffield) > Division of Genomic Medicine (Sheffield) > Department of Oncology and Metabolism (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 25 Aug 2016 15:30 |
Last Modified: | 25 Aug 2016 15:30 |
Published Version: | http://dx.doi.org/10.1098/rsos.150655 |
Status: | Published |
Publisher: | Royal Society |
Refereed: | Yes |
Identification Number: | 10.1098/rsos.150655 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:104102 |