Schuster, C.F., Bellows, L.E., Tosi, T. et al. (4 more authors) (2016) The second messenger c-di-AMP inhibits the osmolyte uptake system OpuC in Staphylococcus aureus. Science Signaling, 9 (441). ra81-ra81. ISSN 1945-0877
Abstract
Staphylococcus aureus is an important opportunistic human pathogen that is highly resistant to osmotic stresses. In order to survive an increase in osmolarity, bacteria immediately take up potassium and small organic compounds, also referred to as compatible solutes. The second messenger c-di-AMP binds to several receptor proteins, most of which are involved in ion and potassium uptake, that help bacteria cope with osmotic stress. In this study, we identified OpuCA, the ATPase component of an uptake system for the compatible solute carnitine, as a cdi-AMP target protein in S. aureus and found that a strain overproducing c-di-AMP showed reduced carnitine uptake. The CBS domains of OpuCA bound to c-di-AMP, and a crystal structure revealed a putative binding pocket for c-di-AMP in the cleft between the two CBS domains. Thus, c-di-AMP is involved in regulating both branches of osmoprotection (potassium uptake and compatible solute uptake), suggesting that c-di-AMP is a general osmotic stress regulator
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 American Association for the Advancement of Science. This is an author produced version of a paper subsequently published in Science Signaling. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 25 Aug 2016 08:51 |
Last Modified: | 13 Jan 2020 14:47 |
Published Version: | http://dx.doi.org/10.1126/scisignal.aaf7279 |
Status: | Published |
Publisher: | American Association for the Advancement of Science |
Refereed: | Yes |
Identification Number: | 10.1126/scisignal.aaf7279 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:104059 |