Marquitan, M, Clausmeyer, J, Actis, P orcid.org/0000-0002-7146-1854 et al. (5 more authors) (2016) Intracellular Hydrogen Peroxide Detection with Functionalised Nanoelectrodes. ChemElectroChem, 3 (12). pp. 2125-2129. ISSN 2196-0216
Abstract
Hydrogen peroxide (H2O2) is one of the most important reactive oxygen species and it is involved in a number of cellular processes ranging from signal transduction to immune-defence and oxidative stress. It is of great interest to intracellularly quantify H2O2 to improve the understanding of its role in disease processes. In this study, we present an amperometric nanosensor for the quantification of H2O2 at the single-cell level. Deposition of the electrocatalyst Prussian Blue on carbon nanoelectrodes enables the selective H2O2 reduction at mild potentials. Because of their small size and needle-type shape, these nanoelectrodes can penetrate the membrane of single living cells causing only minimal perturbation. The nanosensors allow the monitoring of penetration-induced oxidative outbursts as well as the uptake of H2O2 from the extracellular environment in single murine macrophages.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 WILEY-VCH Verlag GmbH & Co. This is the peer reviewed version of the following article: Marquitan, M., Clausmeyer, J., Actis, P., Córdoba, A. L., Korchev, Y., Mark, M. D., Herlitze, S. and Schuhmann, W. (2016), Intracellular Hydrogen Peroxide Detection with Functionalised Nanoelectrodes. CHEMELECTROCHEM.; which has been published in final form at https://dx.doi.org/10.1002/celc.201600390. This article may be used for non-commercial purposes in accordance with the Wiley Terms and Conditions for Self-Archiving. |
Keywords: | biosensors; hydrogen peroxide detection; nanoelectrode; reactive oxygen species; single-cell analysis |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 31 Aug 2016 11:52 |
Last Modified: | 14 Aug 2017 08:06 |
Published Version: | https://dx.doi.org/10.1002/celc.201600390 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/celc.201600390 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:103995 |