Crowther, GS, Wilcox, MH and Chilton, CH orcid.org/0000-0002-8076-1699 (2016) An In Vitro Model of the Human Colon: Studies of Intestinal Biofilms and Clostridium difficile Infection. Methods in Molecular Biology, 1476. pp. 223-234. ISSN 1064-3745
Abstract
The in vitro gut model is an invaluable research tool to study indigenous gut microbiota communities, the behavior of pathogenic organisms, and the therapeutic and adverse effect of antimicrobial administration on these communities. The model has been validated against the intestinal contents of sudden death victims to reflect the physicochemical and microbiological conditions of the proximal to distal colon, and has been extensively used to investigate the interplay between gut microbiota populations, antibiotic exposure, and Clostridium difficile infection. More recently the gut model has been adapted to additionally model intestinal biofilm. Here we describe the structure, assembly, and application of the biofilm gut model.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016, Springer. This is an author produced version of a paper published in Methods in Molecular Biology. Uploaded in accordance with the publisher's self-archiving policy. The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-6361-4_17. |
Keywords: | Chemostat; Clostridium difficile; Biofilm; Modeling; Mucosa; Microbiota |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Molecular Gastroenterology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 19 Aug 2016 10:19 |
Last Modified: | 15 Aug 2017 20:28 |
Published Version: | http://dx.doi.org/10.1007/978-1-4939-6361-4_17 |
Status: | Published |
Publisher: | Springer New York |
Identification Number: | 10.1007/978-1-4939-6361-4_17 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:103868 |